Category | Specifications |
Brand | BIONOON |
Application Fields | Environmental Protection, Food/Agricultural Products, Chemical Industry, Bio-industry, Pharmaceutical/Biopharmaceutical |
Sample Processing Capacity | 32samples |
Adjustable Amplitude | 1-99.9% |
Data Storage | 20 groups (of experimental parameters) |
Cooling Temperature Range | -5℃ to room temperature |
Nucleic Acid Sample Processing Range | 100bp-1kbp |
Time Control | Working time: 0.1s-999min (freely adjustable) |
Adjustable Power | 1-99.9% (Total power: 1000W) |
I. Product Introduction
The BIONOON-32P Non-Contact High-Throughput DNA Fragmenter uses an isothermal, non-contact method to fragment, homogenize, and mix up to 32 samples at once—all through closed centrifuge tubes. It is suitable for sterile, ultra-microvolume disruption and chromosome fragmentation, and is specifically designed for sample pretreatment in next-generation sequencing (NGS) DNA samples and Chromatin Immunoprecipitation (ChIP) assays. It can process as little as 5 μL of ultra-microvolume samples, making it ideal for precious samples.
II. Product Features
1. High Throughput: Processes up to 32 samples simultaneously in a single run, ensuring high experimental efficiency.
2. Cross-Contamination Prevention: No frequent probe operation is required; each sample is contained in a separate, fully closed test tube to avoid cross-contamination. Meanwhile, the sealed environment effectively prevents aerosols or foams generated during sonication from spreading into the surroundings, eliminating potential biological hazards.
3. Excellent Repeatability & High Accuracy: Ultrasonic parameters are flexibly adjustable, and experimental procedures are standardized—ensuring good experimental repeatability and reliable results.
4. Superior Sample Protection: Adopts 4°C low-temperature water bath sonication, which enables uniform energy distribution and effective sonication. The low-temperature environment prevents sample denaturation.
5. Rich Configurations: Compatible with multiple adapters (0.1mL–15mL) to meet the experimental needs of various sample volumes.
6. Low Experimental Cost: No special proprietary consumables are required, resulting in relatively low experimental costs.
III. Working Principle
Through a piezoelectric transducer, electrical signals are converted into high-frequency ultrasonic signals, which generate millions of molecular "cavitation bubbles." The transient hydrodynamic shear force produced when these "cavitation bubbles" expand and burst within seconds is used to fragment, homogenize, and mix samples in an isothermal, non-contact manner.
IV. Application Fields
1. DNA fragmentation
2. RNA fragmentation
3. Sample pretreatment for ChIP assay (Chromatin Immunoprecipitation)
4. Disruption of bacteria and cells, and extraction of membrane proteins
5. Disruption, homogenization, and emulsification of mold spores, etc.
6. Ultrasonic treatment of valuable reagents
7. Sample pretreatment for high-throughput sequencing
V. Technical Parameters
1. Adopts the ultrasonic focusing principle for sample disruption.
2. Samples remain closed throughout the process of bacterial and cell disruption, avoiding cross-contamination.
3. Suitable for ultra-microvolume EP tubes; can process 5 μL samples, making it ideal for precious samples.
4. Applicable for sample pretreatment of sequencing DNA samples and ChIP (Chromatin Immunoprecipitation) assays.
5. Nucleic acid sample processing range: 100bp–1kbp.
6. Samples do not come into contact with the ultrasonic probe; disruption is performed in closed tubes throughout the process.
7. Control method: Chinese-English synchronous touchscreen operation; real-time display of phase, frequency, and waveform.
8. Control screen size: 4.3-inch touchscreen.
9. Frequency control: Automatic tracking.
10. Processing capacity: Up to 32 samples (200 μL each)
VI. Configuration List
1. One main unit (including a cooling circulation system);
2. Two of the following adapters are optional:
200ul adapter/Can process up to 32 samples at a time;
500ul adapter/can process up to 16 samples at a time;
1.5/2ml adapter/Can process up to10 samples at a time;
5ml adapter/can process up to 5 samples at a time.
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